2ST1

THE THREE-DIMENSIONAL STRUCTURE OF BACILLUS AMYLOLIQUEFACIENS SUBTILISIN AT 1.8 ANGSTROMS AND AN ANALYSIS OF THE STRUCTURAL CONSEQUENCES OF PEROXIDE INACTIVATION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	39.250, 72.860, 75.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.144
RMSD bond length	0.015
RMSD bond angle	2.500
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.040 *
Total number of observations	15461 *
Number of reflections	15461 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3-5 (mg/ml)
2	1	drop		27 (%sat)
3	1	drop	sodium acetate	0.5 (M)
4	1	drop		10 (mM)
5	1	drop	phenylmethylsulfonyl fluoride
6	1	reservoir		40 (%sat)