2SPT

DIFFERENCES IN THE METAL ION STRUCTURE BETWEEN SR-AND CA-PROTHROMBIN FRAGMENT 1

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	39.690, 54.120, 131.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.500
R-factor	0.167
RMSD bond length	0.013
RMSD bond angle	0.050
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Number of reflections	5331 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		taken from Soriano-Garacia, M. et al (1989), Biochemistry, 28, 6805-6810. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cacodylate	0.1 (M)
2	1	drop	PEG6000	8 (%(w/v))
3	1	drop		100 (M)
4	1	reservoir	cacodylate	0.1 (M)
5	1	reservoir	PEG6000	16 (%(w/v))
6	1	reservoir		200 (M)