2SPO

A NOVEL SITE-DIRECTED MUTANT OF MYOGLOBIN WITH AN UNUSUALLY HIGH O2 AFFINITY AND LOW AUTOOXIDATION RATE

Experimental procedure

Spacegroup name	P 6
Unit cell lengths	91.200, 91.200, 45.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 1.700
R-factor	0.157
Rwork	0.157
RMSD bond length	0.016
RMSD bond angle	2.080
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.155 *
Total number of observations	68019 *
Number of reflections	20549 *
Completeness [%]	84.9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	ammonium sulfate	2.6-2.8 (M)
3	1	reservoir	Tris-HCl	20 (mM)
4	1	reservoir	EDTA	1 (mM)