2SPL
A NOVEL SITE-DIRECTED MUTANT OF MYOGLOBIN WITH AN UNUSUALLY HIGH O2 AFFINITY AND LOW AUTOOXIDATION RATE
Experimental procedure
| Spacegroup name | P 6 |
| Unit cell lengths | 91.200, 91.200, 45.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 1.700 |
| R-factor | 0.152 |
| Rwork | 0.152 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.595 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2.6-2.8 (M) | |
| 3 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | EDTA | 1 (mM) |
