2SNM

IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	47.156, 47.156, 62.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.970
R-factor	0.183
RMSD bond length	0.011 *
RMSD bond angle	2.094 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.370
High resolution limit [Å]	1.970	1.970 *
Rmerge	0.035 *
Total number of observations	47042 *
Number of reflections	9111 *
Completeness [%]	91.0 *	60 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MPD	57 (%)
2	1	1	Tris-HCl	200 (mM)