2SKE

PYRIDOXAL PHOSPHORYLASE B IN COMPLEX WITH PHOSPHITE, GLUCOSE AND INOSINE-5'-MONOPHOSPHATE

Replaces:  1SKE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	293
Detector technology	FILM
Collection date	1993-10-21
Spacegroup name	P 43 21 2
Unit cell lengths	128.500, 128.500, 116.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.880 - 2.460
R-factor	0.168
Rwork	0.168
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2prj
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	MOSCO
Data scaling software	CCP4
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.880	2.570
High resolution limit [Å]	2.460	2.460
Rmerge	0.074
Total number of observations	98321 *
Number of reflections	30529
Completeness [%]	84.9	65
Redundancy	3.039

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.7	16 *	T-STATE PYRIDOXAL PHOSPHORYLASE B, FROM RABBIT MUSCLE, WAS CO-CRYSTALLIZED WITH 8-10 MM FLUOROPHOSPHATE IN A MEDIUM CONSISTING OF 15-25 MG/ML ENZYME, 2 MM IMP, 2 MM SPERMINE, 50 MM GLUCOSE, 10 MM BES, 0.1 MM EDTA, AND 0.02% SODIUM AZIDE, PH 6.7 (16 DEGREE C). JUST BEFORE DATA COLLECTION, THE CRYSTALS WERE TRANSFERRED TO A FRESH BUFFER SOLUTION, CONTAINING THE SAME CONSTITUENTS AS THEIR MOTHER LIQUOR WITH THE EXCEPTION OF THE PROTEIN., VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SPERMINE
2	1	1	BES
3	1	1	EDTA
4	1	1	NAN3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1		phosphate	8-10 (mM)
2	1		phosphite	8-10 (mM)
3	1		enzyme	15-25 (mg/ml)
4	1		IMP	2 (mM)
5	1	1	spermine	2 (mM)
6	1	1	Glc	50 (mM)
7	1	1	Bes	10 (mM)
8	1	1	EDTA	0.1 (mM)
9	1	1	sodium azide	0.02 (%)