2SKD

PYRIDOXAL PHOSPHORYLASE B IN COMPLEX WITH PHOSPHATE, GLUCOSE AND INOSINE-5'-MONOPHOSPHATE

Replaces:  1SKD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	293
Detector technology	FILM
Collection date	1993-10-21
Spacegroup name	P 43 21 2
Unit cell lengths	128.500, 128.500, 116.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.730 - 2.400
R-factor	0.167
Rwork	0.167
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2prj
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	MOSCO
Data scaling software	CCP4
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.730	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.064
Total number of observations	103280 *
Number of reflections	30041
Completeness [%]	78.1	20.2
Redundancy	3.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.7	16 *	T-STATE PYRIDOXAL PHOSPHORYLASE B, FROM RABBIT MUSCLE, WAS CO-CRYSTALLIZED WITH 8-10 MM FLUOROPHOSPHATE IN A MEDIUM CONSISTING OF 15-25 MG/ML ENZYME, 2 MM IMP, 2 MM SPERMINE, 50 MM GLUCOSE, 10 MM BES, 0.1 MM EDTA, AND 0.02% SODIUM AZIDE, PH 6.7 (16 DEGREE C). JUST BEFORE DATA COLLECTION, THE CRYSTALS WERE TRANSFERRED TO A FRESH BUFFER SOLUTION, CONTAINING THE SAME CONSTITUENTS AS THEIR MOTHER LIQUOR WITH THE EXCEPTION OF THE PROTEIN., temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SPERMINE
2	1	1	GLUCOSE
3	1	1	BES
4	1	1	EDTA
5	1	1	NAN3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1		phosphate	8-10 (mM)
2	1		phosphite	8-10 (mM)
3	1		enzyme	15-25 (mg/ml)
4	1		IMP	2 (mM)
5	1		spermine	2 (mM)
6	1	1	Glc	50 (mM)
7	1	1	Bes	10 (mM)
8	1	1	EDTA	0.1 (mM)
9	1	1	sodium azide	0.02 (%)