2SCU
A detailed description of the structure of Succinyl-COA synthetase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-01 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 98.680, 98.680, 403.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| Rwork | 0.195 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.042 |
| Data reduction software | WEIS |
| Data scaling software | BIOMOL ((KBRANI) |
| Refinement software | TNT (5EB) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.500 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.450 |
| Total number of observations | 417950 * | |
| Number of reflections | 83411 * | |
| Completeness [%] | 92.7 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.3 * | 21 * | Wolodko, W.T., (1984) J. Biol. Chem., 259, 5316. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 10 (mg/ml) | |
| 2 | 1 | 2 | ammonium sulfate | 1.9 (M) | |
| 3 | 1 | 2 | potassium phosphate | 0.1 (M) | |
| 4 | 1 | 2 | dithiothreitol | 1 (mM) | |
| 5 | 1 | 2 | CoA | 0.1 (mM) | |
| 6 | 1 | 2 | EDTA | 0.1 (mM) |
