Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 144.900, 144.900, 109.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.000 - 2.600 |
| Rwork | 0.208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ltn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 20.370 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 90.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.126 |
| Number of reflections | 17287 |
| Completeness [%] | 81.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 | 4 * | 0.1 M HEPES, PH 7.2; 1 MM CACL2; 1 MM MNCL2 0.5 M NACL |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 2 | 0.1 M HEPES, PH 7.2 | ||
| 2 | 1 | 2 | 1 MM CACL2 | ||
| 3 | 1 | 2 | 1 MM MMCL2 | ||
| 4 | 1 | 2 | 0.5 M NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SBA | 34 (mg/ml) | |
| 2 | 1 | drop | sugar | 0.755 (M) | |
| 3 | 1 | drop | HEPES | 0.1 (M) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | 0.15 (M) | ||
| 7 | 1 | reservoir | HEPES | 0.1 (M) | |
| 8 | 1 | reservoir | 0.5 (M) |
