2RVE
THE CRYSTAL STRUCTURE OF ECORV ENDONUCLEASE AND OF ITS COMPLEXES WITH COGNATE AND NON-COGNATE DNA SEGMENTS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.500, 79.600, 66.400 |
Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
Resolution | 6.000 - 3.000 |
R-factor | 0.178 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.750 * |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.047 * |
Total number of observations | 32155 * |
Number of reflections | 17027 * |
Completeness [%] | 99.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | Winkler, F.K., (1991) J.Mol.Biol., 217, 235. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | PEG 4000 | ||
3 | 1 | 1 | NACL | ||
4 | 1 | 2 | WATER | ||
5 | 1 | 2 | MES | ||
6 | 1 | 2 | NACL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 7-10 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | ||
3 | 1 | reservoir | sodium potassium phosphate | 10-20 (mM) | |
4 | 1 | reservoir | 250 (mM) | ||
5 | 1 | reservoir | oligonucleotide | 2-6 (mg/ml) |