2RTN

STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 2.0, SPACE GROUP I222

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 2 2 2
Unit cell lengths	95.410, 106.540, 48.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.800
R-factor	0.276
Rwork	0.276
R-free	0.20000
RMSD bond length	0.014
RMSD bond angle	27.100 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.880
High resolution limit [Å]	1.340 *	1.800
Rmerge	0.064
Total number of observations	82811 *
Number of reflections	31257
Completeness [%]	69.0	27.1
Redundancy	2.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)
4	1	reservoir	ammonium sulfate	30 (%sat)