2RTM
STREPTAVIDIN-2-IMINOBIOTIN-SULFATE COMPLEX, PH 3.50, SPACE GROUP I4122
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 58.170, 58.170, 174.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 1.330 * |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.23800 |
RMSD bond length | 0.017 |
RMSD bond angle | 24.700 * |
Data reduction software | bioteX ((MSC)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.650 | |
High resolution limit [Å] | 1.330 * | 1.580 |
Rmerge | 0.045 | |
Total number of observations | 63959 * | |
Number of reflections | 28990 | |
Completeness [%] | 65.0 | 31.7 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 * | 20 * | Pahler, A., (1987) J. Biol. Chem., 262, 13933. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-20 (mg/ml) | |
2 | 1 | drop | potassium acetate | 50 (mM) | |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | reservoir | ammonium sulfate | 30 (%sat) |