2RTC

APOSTREPTAVIDIN, PH 3.60, SPACE GROUP I222

Experimental procedure

Temperature [K]	273
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	I 2 2 2
Unit cell lengths	95.180, 105.390, 47.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.500
R-factor	0.21
Rwork	0.210
R-free	0.26500
RMSD bond length	0.019
RMSD bond angle	24.600 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.320 *
Rmerge	0.061
Total number of observations	75219 *
Number of reflections	40370
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)
4	1	reservoir	ammonium sulfate	30 (%sat)