2RNF

X-RAY CRYSTAL STRUCTURE OF HUMAN RIBONUCLEASE 4 IN COMPLEX WITH D(UP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	32.830, 94.330, 40.660
Unit cell angles	90.00, 107.20, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.16
Rwork	0.160
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rat
RMSD bond length	0.011
RMSD bond angle	1.512
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.134
Total number of observations	36143 *
Number of reflections	7980 *
<I/σ(I)>	7.8	2.76
Completeness [%]	85.8	89.2
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.3 *		pH 6.9

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM CACODYLATE
2	1	1	PEG 4000
3	1	2	PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	30 (%(v/v))
3	1	reservoir	sodium cacodylate	0.1 (M)