2RMC

Crystal structure of murine cyclophilin C complexed with immunosuppressive drug cyclosporin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 1 21 1
Unit cell lengths	60.300, 74.000, 97.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.640
R-factor	0.197
Rwork	0.197
RMSD bond length	0.012
RMSD bond angle	2.680
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.640 *
Rmerge	0.040 *
Total number of observations	139812 *
Number of reflections	73850 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	complex	13 (mg/ml)
10	1	2	CsA	0.01 (mM)
2	1	2	Tris	20 (mM)
3	1	2	dithiothreitol	2 (mM)
4	1	2	EDTA	2 (mM)
5	1	2		0.5 (mM)
6	1	2	PEG3350	14 (%(w/v))
7	1	2	ethanol	6 (%(v/v))
8	1	2	isopropanol	2 (%(v/v))
9	1	2	ammonium sulfate	0.2 (M)