2RLA
ALTERING THE BINUCLEAR MANGANESE CLUSTER OF ARGINASE DIMINISHES THERMOSTABILITY AND CATALYTIC FUNCTION
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1995-01 |
Detector | RIGAKU |
Spacegroup name | P 32 |
Unit cell lengths | 88.500, 88.500, 106.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RAT LIVER ARGINASE |
RMSD bond length | 0.010 |
RMSD bond angle | 23.700 * |
Data reduction software | MOSFLM |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.064 | 0.400 |
Total number of observations | 17377 * | |
Number of reflections | 14894 | |
<I/σ(I)> | 4.3 | 1.6 |
Completeness [%] | 80.2 | 79.7 |
Redundancy | 2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | 12 - 18% PEG 8000, 50 MM BICINE PH = 8.5, 0.05% AZIDE, 1 MM MNCL2 SOAKED FOR 1 WEEK WITH 20 MM EDTA + DPA |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | drop | bicine | 50 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
5 | 1 | reservoir | bicine | 50 (mM) | |
6 | 1 | reservoir | 1 (mM) | ||
7 | 1 | reservoir | sodium azide | 0.05 (%(w/v)) |