2RL3
Crystal structure of the OXA-10 W154H mutant at pH 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.680, 97.060, 125.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.680 - 1.900 |
| R-factor | 0.17109 |
| Rwork | 0.169 |
| R-free | 0.21815 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4f |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.497 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.123 | 0.762 |
| Number of reflections | 47547 | |
| <I/σ(I)> | 10.9 | 1.7 |
| Completeness [%] | 99.4 | 96.2 |
| Redundancy | 5.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.6M AS, 0.1M HEPES, PEG 30%, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
