2RL0
Crystal structure of the fourth and fifth fibronectin F1 modules in complex with a fragment of staphylococcus aureus fnbpa-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.580, 85.580, 230.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.160 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Previous low resolution structure |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.552 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.161 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.300 |
| Total number of observations | 42415 | |
| Number of reflections | 58383 | |
| <I/σ(I)> | 22.5 | 3.8 |
| Completeness [%] | 99.1 | 97.5 |
| Redundancy | 7.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 0.2M NACL, 0.1M BIS TRIS, 14% PEG3350, pH6.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
