2RKX
The 3D structure of chain D, cyclase subunit of imidazoleglycerol_evolvedcerolphosphate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 96.380, 96.380, 153.988 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.20716 |
| Rwork | 0.206 |
| R-free | 0.23481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1thf |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.740 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.098 | 0.435 |
| Number of reflections | 20784 | |
| <I/σ(I)> | 36.6 | 6.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.7 | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7 | 292 | 20% PEG 3350, 0.1M Bis-Tris propane pH 8.5, 0.2M NaF, pH 7.0, MICROBATCH UNDER OIL, temperature 292K |
