2RKQ
Crystal structure of drosophila peptidoglycan recognition protein SD (PGRP-SD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.559, 57.438, 71.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.15567 |
| Rwork | 0.155 |
| R-free | 0.17328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.363 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 25380 | |
| <I/σ(I)> | 10 | 3.8 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 25% PEG-mme 2000, 0.1M MES , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
