2RKO

Crystal Structure of the Vps4p-dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	109.116, 109.116, 176.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.350
R-factor	0.27
Rwork	0.268
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xwi
RMSD bond length	0.008
RMSD bond angle	1.107
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.498	94.500	3.530
High resolution limit [Å]	3.350	10.590	3.350
Rmerge	0.057	0.019	0.440
Total number of observations		1749	11338
Number of reflections	9446
<I/σ(I)>	22.6	26.9	4.9
Completeness [%]	99.4	82.5	100
Redundancy	8.1	5.7	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.9	292	0.1M MES, 0.8M-1.9M MgSO4, pH 5.9-6.5, VAPOR DIFFUSION, temperature 292K