2RKC

Crystal structure of the measles virus hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.072, 1.072
Spacegroup name	P 41 21 2
Unit cell lengths	134.197, 134.197, 100.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.700
Rwork	0.222
R-free	0.25140
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.452
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.083	0.538
Number of reflections	25665
<I/σ(I)>	15	2.9
Completeness [%]	98.8	99.9
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.2M sodium dihydrogen phosphate, 0.3M di-potassium hydrogen phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 295K