2RKB

Serine dehydratase like-1 from human cancer cells

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2007-01-01
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	97.210, 154.740, 306.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.800
R-factor	0.213
Rwork	0.213
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Phasing software	CNS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.157
<I/σ(I)>	10
Completeness [%]	100.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.6	294	50 mM Na-citrate buffer, 10 mM O-methylserine, 200 mM potassium acetate, 5 mM DTT, 15 % PEG-8000, 10 mg/mL protein, pH 5.6, EVAPORATION, temperature 294K