2RK9
The crystal structure of a glyoxalase/bleomycin resistance protein/dioxygenase superfamily member from Vibrio splendidus 12B01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.704, 76.954, 42.395 |
Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
Resolution | 40.680 - 1.600 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.22900 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.060 | 0.167 |
Number of reflections | 32833 | |
<I/σ(I)> | 16 | 5.5 |
Completeness [%] | 94.6 | 65.7 |
Redundancy | 6.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.2 M NaCl, 0.1 M Sodium acetate pH 4.6, 30% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 294K |