2RK7
The Structure of rat cytosolic PEPCK in complex with oxalate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-19 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.011, 118.945, 86.506 |
Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
Resolution | 30.820 - 1.900 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.25600 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.119 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.130 | 0.062 | 0.680 |
Number of reflections | 96505 | ||
<I/σ(I)> | 7.9 | ||
Completeness [%] | 99.2 | 98 | 96.3 |
Redundancy | 5.5 | 5.9 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 15-30% PEG 3350, 0.1M HEPES,10 MM MNCL2, pH 7.4, temperature 293K, VAPOR DIFFUSION, HANGING DROP |