2RK4
Structure of M26I DJ-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-04-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.827 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.770, 74.770, 75.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.150 |
R-factor | 0.1294 |
Rwork | 0.129 |
R-free | 0.15620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p5f |
RMSD bond length | 0.014 |
RMSD bond angle | 0.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.068 | 0.560 |
Number of reflections | 86085 | |
<I/σ(I)> | 23.4 | 2.3 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 6.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30% PEG 3000, 100 mM HEPES, 200 mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |