2RJZ
Crystal structure of the type 4 fimbrial biogenesis protein PilO from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 64 |
| Unit cell lengths | 70.471, 70.471, 116.876 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.458 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.085 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.132 | 0.557 |
| Number of reflections | 16706 | |
| <I/σ(I)> | 11.8 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 100 mM Tris-HCl pH 8.0, 2 M Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |
