2RJO

Crystal structure of Twin-arginine translocation pathway signal protein from Burkholderia phytofirmans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	51.197, 51.504, 138.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.200 - 2.050
R-factor	0.234
Rwork	0.234
R-free	0.28100
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.040	2.040
Rmerge	0.070	0.351
Number of reflections	20727
<I/σ(I)>	8.6	1
Completeness [%]	85.1	44.2
Redundancy	10.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M Ammonium sulfate, 25% PEG 8000, 1% D-galactose, VAPOR DIFFUSION, SITTING DROP, temperature 277K