2RJI

Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.977
Spacegroup name	C 2 2 21
Unit cell lengths	50.237, 65.591, 105.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.840 - 1.800
R-factor	0.21003
Rwork	0.208
R-free	0.24114
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.247
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.040	0.357
Number of reflections	16406
<I/σ(I)>	25.3	3.7
Completeness [%]	99.3	99.7
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	0.1M citric acid, 1M LiCl, 25-30% PEG 6K, pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	0.1M citric acid, 1M LiCl, 25-30% PEG 6K, pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K