2RJ9
B-specific alpha-1,3-galactosyltransferase (GTB) + UDP+ Amino-deoxy-acceptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-15 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.430, 149.930, 78.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.690 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.612 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.830 | 19.830 | 1.750 |
| High resolution limit [Å] | 1.690 | 3.630 | 1.690 |
| Rmerge | 0.044 | 0.024 | 0.325 |
| Total number of observations | 17298 | 13851 | |
| Number of reflections | 34204 | ||
| <I/σ(I)> | 16.2 | 47.7 | 3.5 |
| Completeness [%] | 97.0 | 94.8 | 98.4 |
| Redundancy | 4.71 | 4.9 | 3.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
