2RJ8
B-specific alpha-1,3-galactosyltransferase (GTB) +UDP+ H-antigen disaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-17 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.450, 149.600, 78.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.690 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.413 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.4SSI) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 19.790 | 1.750 |
High resolution limit [Å] | 1.690 | 3.630 | 1.690 |
Rmerge | 0.048 | 0.026 | 0.324 |
Total number of observations | 18511 | 14640 | |
Number of reflections | 34923 | ||
<I/σ(I)> | 15.1 | 43.6 | 3.5 |
Completeness [%] | 99.5 | 96.9 | 99.9 |
Redundancy | 4.87 | 5.09 | 4.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |