2RJ2

Crystal Structure of the Sugar Recognizing SCF Ubiquitin Ligase at 1.7 Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-11-23
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	61.760, 61.760, 115.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.910 - 1.700
R-factor	0.18834
Rwork	0.185
R-free	0.21482
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1umh
RMSD bond length	0.012
RMSD bond angle	1.304
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.790
High resolution limit [Å]	1.700	1.700
Number of reflections	27682
Completeness [%]	97.4	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1M TRIS, 0.1%(v/v) PEG 400, 0.01M NICKEL CHLORIDE, 1.7M AMMONIUM SULFATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K