2RJ0
B-specific alpha-1,3-galactosyltransferase G176R mutant + UDP+ Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-07 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.530, 149.350, 79.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.520 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 19.910 | 1.570 |
| High resolution limit [Å] | 1.520 | 3.270 | 1.520 |
| Rmerge | 0.042 | 0.020 | 0.355 |
| Total number of observations | 44512 | 31057 | |
| Number of reflections | 48558 | ||
| <I/σ(I)> | 20.1 | 62 | 4.2 |
| Completeness [%] | 99.9 | 100 | 99.4 |
| Redundancy | 7.98 | 8.7 | 6.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
