2RIX
B-specific-1,3-galactosyltransferase)(GTB) + UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.540, 149.930, 79.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.750 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.441 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.830 | 19.830 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.760 | 1.750 |
| Rmerge | 0.051 | 0.025 | 0.338 |
| Total number of observations | 22263 | 18051 | |
| Number of reflections | 31525 | ||
| <I/σ(I)> | 16.7 | 47.2 | 3.9 |
| Completeness [%] | 98.5 | 100 | 96.2 |
| Redundancy | 6.59 | 6.6 | 5.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
