2RIX
B-specific-1,3-galactosyltransferase)(GTB) + UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2006-08-01 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.540, 149.930, 79.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 1.750 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.441 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.4SSI) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.830 | 19.830 | 1.810 |
High resolution limit [Å] | 1.750 | 3.760 | 1.750 |
Rmerge | 0.051 | 0.025 | 0.338 |
Total number of observations | 22263 | 18051 | |
Number of reflections | 31525 | ||
<I/σ(I)> | 16.7 | 47.2 | 3.9 |
Completeness [%] | 98.5 | 100 | 96.2 |
Redundancy | 6.59 | 6.6 | 5.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |