2RIT
Unliganded B-specific-1,3-galactosyltransferase (GTB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-26 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.535, 149.906, 79.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.430 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.284 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 19.800 | 1.480 |
| High resolution limit [Å] | 1.430 | 3.080 | 1.430 |
| Rmerge | 0.034 | 0.019 | 0.322 |
| Total number of observations | 28569 | 17965 | |
| Number of reflections | 57030 | ||
| <I/σ(I)> | 19.1 | 61.8 | 3.4 |
| Completeness [%] | 98.1 | 92.8 | 96.9 |
| Redundancy | 4.22 | 5.01 | 3.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
