2RIM
Crystal structure of Rtt109
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.401, 69.233, 55.661 |
| Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
| Resolution | 46.200 - 2.200 |
| R-factor | 0.21864 |
| Rwork | 0.216 |
| R-free | 0.25907 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.080 | 2.200 |
| Number of reflections | 30288 | |
| Completeness [%] | 1.0 | 0.971 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG550 MME, Ammonium citrate, glycerol, Tris, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
