2RIK
I-band fragment I67-I69 from titin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.686, 86.294, 44.698 |
| Unit cell angles | 90.00, 104.97, 90.00 |
Refinement procedure
| Resolution | 18.970 - 1.600 |
| Rwork | 0.171 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | trimmed poly-Ala model of 1TLK |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.507 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.395 |
| Number of reflections | 41644 | |
| <I/σ(I)> | 15.25 | 3.5 |
| Completeness [%] | 99.4 | 87.9 |
| Redundancy | 4.57 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | pH 4.6, vapor diffusion, hanging drop, temperature 298K |
