2RIJ
Crystal structure of a putative 2,3,4,5-tetrahydropyridine-2-carboxylate n-succinyltransferase (cj1605c, dapd) from campylobacter jejuni at 1.90 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97957, 0.95373, 0.97942 |
Spacegroup name | H 3 2 |
Unit cell lengths | 135.093, 135.093, 213.742 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.540 - 1.900 |
R-factor | 0.157 |
Rwork | 0.156 |
R-free | 0.18500 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.550 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.540 | 29.540 | 1.950 |
High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
Rmerge | 0.124 | 0.011 | 0.011 |
Total number of observations | 4835 | 32463 | |
Number of reflections | 59121 | ||
<I/σ(I)> | 4.1 | 6.2 | 0.7 |
Completeness [%] | 100.0 | 97.5 | 100 |
Redundancy | 7.5 | 6.8 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | NANODROP, 1.0M Na Citrate, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |