2RI8
Penicillium citrinum alpha-1,2-mannosidase complex with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.487, 110.997, 86.235 |
| Unit cell angles | 90.00, 99.17, 90.00 |
Refinement procedure
| Resolution | 20.320 - 2.160 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.083 | 0.278 |
| Number of reflections | 52973 | |
| <I/σ(I)> | 10.6 | 4.3 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3.8 | 3.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15-18% PEG 6000, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
