2RHH
Synthetic Gene Encoded Bacillus Subtilis FtsZ with Bound Sulfate Ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.370, 66.370, 152.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.001 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.001 | 2.001 |
| Rmerge | 0.087 | 0.449 |
| Number of reflections | 23413 | |
| <I/σ(I)> | 19.8 | 1.4 |
| Completeness [%] | 98.3 | 88.6 |
| Redundancy | 11.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 50% PEG 200, 0.1M Tris, 50mM lithium sulfate, pH 7.0, vapor diffusion, temperature 293K |
