2RHC
Actinorhodin ketordeuctase, actKR, with NADP+ and Inhibitor Emodin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 103.875, 103.875, 123.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.250 - 2.100 |
| Rwork | 0.186 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.250 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | 0.481 |
| Number of reflections | 43997 | |
| <I/σ(I)> | 30.9 | 5.3 |
| Completeness [%] | 95.7 | 100 |
| Redundancy | 11 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.8-4.8 M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
