2RHA
Crystal structure of a predicted dna-binding transcriptional regulator (saro_1072) from novosphingobium aromaticivorans dsm at 2.10 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-26 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.837, 102.837, 111.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.185 - 2.100 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2QTQ chain A |
RMSD bond length | 0.017 |
RMSD bond angle | 1.649 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.185 | 29.185 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.081 | 0.030 | 0.816 |
Total number of observations | 1742 | 12508 | |
Number of reflections | 35346 | ||
<I/σ(I)> | 6.8 | 20.2 | 0.9 |
Completeness [%] | 99.8 | 93.8 | 100 |
Redundancy | 4.8 | 4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | NANODROP, 1.6M (NH4)2SO4, 0.1M Citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |