2RH8
Structure of apo anthocyanidin reductase from vitis vinifera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-20 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.470, 50.470, 440.406 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.220 |
| R-factor | 0.203 |
| Rwork | 0.199 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c29 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.068 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.727 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.087 | 0.343 |
| Number of reflections | 17253 | |
| <I/σ(I)> | 5.6 | 2 |
| Completeness [%] | 96.4 | 98.8 |
| Redundancy | 11.7 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | NH4 acetate 150 mM, PEG 3350, Hepes 100 mM, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50 |
