2RGO
Structure of Alpha-Glycerophosphate Oxidase from Streptococcus sp.: A Template for the Mitochondrial Alpha-Glycerophosphate Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9296 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.363, 106.785, 58.887 |
| Unit cell angles | 90.00, 99.18, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.400 |
| R-factor | 0.23808 |
| Rwork | 0.238 |
| R-free | 0.24708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rgh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.675 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.890 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.298 | |
| Number of reflections | 54822 | |
| <I/σ(I)> | 9.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 1000, Tris, magnesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
