2RGM
Rice BGlu1 beta-glucosidase, a plant exoglucanase/beta-glucosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2006-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98008 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.287, 101.286, 127.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.210 - 1.550 |
R-factor | 0.17272 |
Rwork | 0.171 |
R-free | 0.19672 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cbg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.402 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.052 | 0.403 |
Number of reflections | 155944 | |
<I/σ(I)> | 17.5 | 2.29 |
Completeness [%] | 98.8 | 96.3 |
Redundancy | 3.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 23% PEG MME 5000, 0.2M ammonium sulfate, 0.1M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 288K |