2RGL
Rice BGlu1 beta-glucosidase, a plant exoglucanase/beta-glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2006-04-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98008 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.051, 100.667, 127.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.670 - 2.200 |
| R-factor | 0.16423 |
| Rwork | 0.162 |
| R-free | 0.20474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.308 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.132 | 0.404 |
| Number of reflections | 53040 | |
| <I/σ(I)> | 15.5 | 5.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 23% PEG MME 5000, 0.17M ammonium sulfate, 0.1M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
