2RGC

Crystal structure of H-RasQ61V-GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	H 3 2
Unit cell lengths	88.430, 88.430, 132.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.280 - 1.600
R-factor	0.191
Rwork	0.191
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctq
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.800
High resolution limit [Å]	1.600
Number of reflections	26159
Completeness [%]	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20 % PEG 3350, 0.2 M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K