2RG8

Crystal Structure of Programmed for Cell Death 4 Middle MA3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	37.680, 70.060, 110.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.680 - 1.800
R-factor	0.202
Rwork	0.202
R-free	0.25100
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.680
High resolution limit [Å]	1.800
Number of reflections	34012
Completeness [%]	99.5
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	300	200mM sodium acetate, 28%-30% PEG4000, 8% Jeffamine M-600, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	8.5	300	200mM sodium acetate, 28%-30% PEG4000, 8% Jeffamine M-600, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K