2RG4
Crystal structure of the uncharacterized protein Q2CBJ1_9RHOB from Oceanicola granulosus HTCC2516
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.476, 69.476, 164.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.380 - 1.900 |
R-factor | 0.211 |
Rwork | 0.207 |
R-free | 0.27700 |
Structure solution method | SAD |
RMSD bond length | 0.023 |
RMSD bond angle | 2.343 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.076 | 0.035 | 0.668 |
Number of reflections | 70428 | ||
<I/σ(I)> | 9 | ||
Completeness [%] | 98.7 | 99.7 | 98.9 |
Redundancy | 6.2 | 7.1 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.1M Bis-tris, 0.3M Magnesium formate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |