2RG1
Crystal structure of E. coli WrbA apoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-03-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9776 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.850, 75.690, 56.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.310 - 1.850 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r96 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.518 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.061 | 0.250 |
| Number of reflections | 26411 | |
| <I/σ(I)> | 21.1 | 5.55 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 285 | 30% PEG 4000,0.2M magnesium chloride, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
